期刊
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY
卷 58, 期 4, 页码 183-188出版社
ACADEMIC PRESS LTD
DOI: 10.1006/pmpp.2001.0325
关键词
thaumatin-like protein; PR-5 protein; Hordeum vulgare; Drechslera teres; N-terminal sequencing; cDNA sequence analysis
Eight thaumatin-like proteins of barley (Hordeum vulgare L.) (TLP1-TLP8) were detected in Drechslera teres infected leaves by two-dimensional electrophoresis followed by N-terminal microsequencing: four of them are acidic; four are basic proteins. Partial amino acid sequence data were used to generate polymerase chain reaction (PCR) clones employed for the isolation of four novel, nearly full-length cDNA sequences encoding TLP4, TLP6, TLP7, and TLP8. The cDNAs are characterized by sequence sites of very high GC content and they encode proteins with 171-233 amino acid residues. The N-termini of the deduced proteins were preceded by putative signal peptides of 22-25 amino acid residues. With a Clustal W based dendrogram similar sequences were assigned to those we obtained in this work. (C) 2001 Academic Press.
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