期刊
BIOCHEMISTRY
卷 48, 期 33, 页码 7906-7915出版社
AMER CHEMICAL SOC
DOI: 10.1021/bi900084m
关键词
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资金
- National Science Foundation [MCB-0818387]
- American Heart Association [0855392F]
- Louisiana Governor's Biotechnology Initiative
- National Institutes of Health [GM 74888, GM 15431]
Lipoxygenases (LOX) play pivotal roles in the biosynthesis of leukotrienes and other biologically active eicosanoids derived from arachidonic acid. A mechanistic understanding of substrate recognition. when lipoxygenases that recognize the same substrate generate different products, can be used to help guide the design of enzyme-specific inhibitors. We report here the 1.85 angstrom resolution structure of an 8R-lipoxygenase from Plexaura homomalla, an enzyme with a sequence similar to 40% identical to that of human 5-LOX. The structure reveals a U-shaped channel, defined by invariant amino acids, that would allow substrate access to the catalytic iron. We demonstrate that mutations within the channel significantly impact enzyme activity and propose a novel model for substrate binding potentially applicable to other members of this enzyme family.
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