Novel method using phytase for separating soybean β-conglycinin and glycinin

A novel method for separating sogbean beta -conglycinin and glycinin from defatted soymilk by a phytase treatment was developed. Phytase was added to defatted soymilk (1000FYT/100 g of protein) at pH 6.0, and the mixture incubated for 1 h at 40 degreesC, This procedure separated beta -conglycinin and glycinin without seeding a reducing agent or cooling into the soluble and insoluble fractions, respectively. Simultaneously most of the phytate in both proteins was removed.