期刊
CHEMISTRY & BIOLOGY
卷 8, 期 4, 页码 357-368出版社
CURRENT BIOLOGY LTD
DOI: 10.1016/S1074-5521(01)00018-7
关键词
autoimmunity; cell death; crystal structure; drug design; x-ray
Background: Granzyme B, one of the most abundant granzymes in cytotoxic T-lymphocyte (CTL) granules, and members of the caspase (cysteine aspartyl proteinases) family have a unique cleavage specificity for aspartic acid in P1 and play critical roles in the biochemical events that culminate in cell death. Results: We have determined the three-dimensional structure of the complex of the human granzyme B with a potent tetrapeptide aldehyde inhibitor. The Asp-specific S1 subsite of human granzyme B is significantly larger and less charged than the corresponding Asp-specific site in the apoptosis-promoting caspases, and also larger than the corresponding subsite in rat granzyme B. Conclusions: The above differences account for the variation in substrate specificity among granzyme B, other serine proteases and the caspases, and enable the design of specific inhibitors that can probe the physiological functions of these proteins and the disease states with which they are associated. (C) 2001 Published by Elsevier Science Ltd.
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