期刊
BIOCHEMISTRY
卷 48, 期 50, 页码 11834-11836出版社
AMER CHEMICAL SOC
DOI: 10.1021/bi901756n
关键词
-
资金
- National Institutes of Health [R21-CA132700, R21-DA025725, R01-GM090324, R01-GM078450]
A peptide fusion tag and accompanying recombinant capture reagents have been developed oil the basis of the peptide-PDZ domain interaction and affinity clamps, a new class of affinity reagent. This system allows for single-step purification under mild conditions and stable capture of a tagged protein. The subnanomolar affinity, high force resistance (> 30 pN), small size (similar to 25 kDa, approximately one-sixth of the size of IgG), and monomeric nature of the affinity clamp are all superior features for many applications, in particular single-molecule measurements.
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