期刊
BIOCHEMISTRY
卷 48, 期 27, 页码 6495-6501出版社
AMER CHEMICAL SOC
DOI: 10.1021/bi900558g
关键词
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资金
- Royal Society Marsden Fund
- National Research Centre for Growth and Development
- Tertiary Education Commission
Peroxiredoxins (Prxs) are a ubiquitous family of thiol peroxidases that protect cells from peroxides and have a putative role in redox. signaling. In this study, we investigated the redox properties of human Prx 3, a typical 2-Cys Prx that is localized to the mitochondrial matrix. We found that Prx 3 displayed strong reactivity with H2O2, with a competitive kinetic approach generating a second order rate constant of 2 x 10(7) M-1 s(-1). This is considerably higher than typical thiols and similar to values for other mammalian 2-Cys Prxs, In contrast, Prx 3 reacted very slowly with the thiol alkylating agents iodoacetamide and N-ethylmaleimide. Using dithiothreitol redox buffers, we measured the redox potential of Prx 3 of -290 mV. This is similar to the redox potential of mitochondrial thioredoxin 2 and is consistent with optimal operation of Prx 3 in the mitochondrial matrix.
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