期刊
BIOCHEMISTRY
卷 48, 期 46, 页码 10882-10893出版社
AMER CHEMICAL SOC
DOI: 10.1021/bi901506f
关键词
-
资金
- Ministry of Science and Higher Education [NN 3013383 33, PBZ-MNiSW-07/11/2007]
- National Institutes of Health [GM 22939]
Hybridization to RNA is important for many applications, including antisense therapeutics, RNA interference, and microarray screening. Similar thermodynamic stabilities of A-U and G-U base pairs result in difficulties in selective binding to RNA. Moreover, A-U pairs are weaker than G-C pairs so that binding is sometimes weak when many A-U pairs are present. It is known, however, that replacement of uridine with 2-thiouridine significantly improves binding and selectivity. To test for additional improvement of binding and of the specificity for binding A over G, LNA-2-thiouridine was synthesized for the first time and incorporated into many LNA-2'-O-methyl-RNA/RNA duplexes. UV melting was used to measure the thermodynamic effect of replacing 2'-O-methyluridine with 2'-O-methyl-2-thiouridine or LNA-2-thiouridine. The 2-thiouridine usually enhances binding and selectivity, Selectivity is optimized when a single 2-thiouridine is placed at an internal position in a duplex.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据