期刊
BIOCHEMISTRY
卷 48, 期 30, 页码 7261-7270出版社
AMER CHEMICAL SOC
DOI: 10.1021/bi900640x
关键词
-
资金
- National Institutes of Health [RO1 GM084724-05, T32 GM08501, 5T32 GM07616-30S1]
Fucose-alpha(1-2)-galactose [Fuc alpha(1-2)Gal] sugars have been implicated in the molecular mechanisms that underlie neuronal development, learning, and memory, However, an understanding of their precise roles has been hampered by a lack of information regarding Fuc alpha(1-2)Gal glycoproteins. Here, we report the first proteomic studies of this plasticity-relevant epitope. We identify five classes of putative Fuc alpha(1-2)Gal glycoproteins: cell adhesion molecules, ion channels and solute carriers/transporters, ATP-binding proteins, synaptic vesicle-associated proteins, and mitochondrial proteins. In addition, we show that Fuc alpha(1-2)Gal glycoproteins are enriched in the developing mouse olfactory bulb (OB) and exhibit a distinct spatiotemporal expression that is consistent with the presence of a glycocode to help direct olfactory sensory neuron (OSN) axonal pathfinding. We find that expression of Fuc alpha(1-2)Gal sugars in the OB is regulated by the alpha(1-2)fucosyltransferase FUT1. FUT1-deficient mice exhibit developmental defects, including fewer and smaller glomeruli and a thinner olfactory nerve layer, suggesting that fucosylation contributes to OB development. Our findings significantly expand the number of Fuc alpha(1-2)Gal glycoproteins and provide new insights into the molecular mechanisms by which fucosyl sugars contribute to neuronal processes.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据