期刊
PLANT PATHOLOGY
卷 50, 期 2, 页码 174-180出版社
BLACKWELL SCIENCE LTD
DOI: 10.1046/j.1365-3059.2001.00558.x
关键词
AFLP; pathotype; RAPD; variation; wheat stripe rust; wheat yellow rust
Since its initial detection in Australia in 1979, wheat yellow (stripe) rust (Puccinia striiformis f.sp, tritici) has evolved in Australia and New Zealand into more than 20 pathotypes with asserted virulence characteristics. This evolution is believed to have occurred in a stepwise fashion from an original single pathotype, with no subsequent new introductions. A combination of random amplified polymorphic DNA (RAPDs) and amplified fragment length polymorphism; (AFLP's) was used to examine the level of molecular variation in Australian and New Zealand isolates, and to compare this with variation amongst other isolates of P. striiformis. Using 60 RAPD primers on seven Australian isolates representing seven different pathotypes collected between 1979 and 1991, more than 300 potentially polymorphic loci were analysed and no polymorphisms were detected. Using the same primers on two UK isolates, 3% of loci showed a polymorphism. A similar level of polymorphism was found between UK isolates using AFLP primers, and between 5 and 15% of fragments were polymorphic between an isolate from the UK, an isolate from Denmark, and one from Colombia. However, no AFLP polymorphisms were found amongst 14 Australian and New Zealand isolates tested, at over 100 potentially polymorphic loci. The lack of molecular variation in the Australian and New Zealand collection is consistent with the stepwise mutation theory of pathotype evolution from a single introduction.
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