期刊
BIOCHEMISTRY
卷 47, 期 46, 页码 11964-11972出版社
AMER CHEMICAL SOC
DOI: 10.1021/bi801222d
关键词
-
资金
- Ministry of Education, Culture, Sports, Science and Technology
- Novozyme Japan Research Fund
CYP105A1 from Streptomyces griseolus has the capability of converting vitamin D-3 (VD3) to its active form, 1 alpha,25-dihydroxyvitamin D-3 (1 alpha,25(OH)(2)D-3) by a two-step hydroxylation reaction. Our previous structural study has suggested that Arg73 and Arg84 are key residues for the activities of CYP105A1 In this study, we prepared a series of single and double mutants by site-directed mutagenesis focusing on these two residues of CYP105A1 to obtain the hyperactive vitamin D-3 hydroxylase. R84F mutation altered the substrate specificity that gives preference to the 1 alpha-hydroxylation of 25-hydroxyvitamin D-3 over the 25-hydroxylation of 1 alpha-hydroxyvitamin D-3, opposite to the wild type and other mutants. The double mutant R73V/R84A exhibited 435- and 110-fold higher k(cat)/K-m values for the 25-hydroxylation of 1 alpha-hydroxyvitamin D-3 and 1 alpha-hydroxylation of 25-hydroxyvitamin D-3, respectively, compared with the wild-type enzyme. These values notably exceed those of CYP27A1, which is the physiologically essential VD3 hydroxylase. Thus, we successfully generated useful enzymes of altered substrate preference and hyperactivity. Structural and kinetic analyses of single and double mutants suggest that the amino acid residues at positions 73 and 84 affect the location and conformation of the bound compound in the reaction site and those in the transient binding site, respectively.
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