期刊
ANALYTICAL CHEMISTRY
卷 73, 期 7, 页码 1622-1626出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac0004512
关键词
-
资金
- PHS HHS [NIH-R01-H601044] Funding Source: Medline
A monolithic capillary electrophoresis system with integrated on-chip fluorescence detector has been microfabricated on a silicon substrate. Photodiodes in the silicon substrate measure fluorescence emitted from eluting molecules. The device incorporates an on-chip thin-film interference filter that prevents excitation light from inhibiting the fluorescence detection. A transparent AZO conducting ground plane is also used to prevent the high electric fields used for the separation from interfering with the photodiode response. Separations of DNA restriction fragments have been performed in these devices with femtogram detection limits using SYBR Green I intercalating dye.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据