期刊
GLYCOBIOLOGY
卷 11, 期 4, 页码 261-274出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/glycob/11.4.261
关键词
allergy; MALDI-TOF-MS; Lewis a; plant glycoproteins
The N-glycans from 27 plant foodstuffs, including one from a gymnospermic plant and one from a fungus, were prepared by a new procedure and examined by means of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). For several samples, glycan structures were additionally investigated by size-fractionation and revel se-phase high-performance liquid chromatography in conjunction,vith exoglycosidase digests and finally also H-1-nuclear magnetic resonance spectroscopy. The glycans found ranged from the typical vacuolar horseradish peroxidase type and oligomannose to complex Le(a)-carrying structures. Though the common mushroom exclusively contained N-glycans of the oligomannosidic type, all plant foods contained mixtures of the above-mentioned types. Apple, asparagus, avocado, banana, carrot, celery, hazelnut, kiwi, onion, orange, pear, pignoli, strawberry, and walnut were particularly rich in Le(a)-carrying N-glycans. Although traces of Le(a)-containing structures were also present in almond, pistachio, potato, and tomato, no such glycans could be found in cauliflower. Coconut exhibited almost exclusively N-glyclans containing only xylose but no fucose, Oligomannosidic N-glycans dominated in buckwheat and especially in the legume seeds mung bean, pea, peanut, and soybean. Papaya presented a unique set of hybrid type structures partially containing the Le(a) determinant. These results are not only compatible with the hypothesis that the carbohydrate structures are another potential source of immunological cross-reaction between different plant allergens, but they also demonstrate that the Le(a)-type structure is very widespread among plants.
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