期刊
PROCESS BIOCHEMISTRY
卷 36, 期 10, 页码 925-932出版社
ELSEVIER SCI LTD
DOI: 10.1016/S0032-9592(00)00248-X
关键词
bioconversion; alpha-pinene; beta-pinene; flavours; monoterpenes; substrate concentration
A soil pseudomonad capable of metabolizing either alpha- or beta -pinene as sole carbon and energy source was isolated with an enrichment culture. Culture broth in mineral media gave rise to a pine-like aroma during the initial fermentation stage and a fresh rose-like aroma during the late fermentation stage. No growth inhibition was found up to 10% pinenes concentration. The bioconversion products of cx-pinene were identified as limonene, p-cymene, alpha -terpinolene, camphor, terpinen-4-ol, a-terpineol, endo-borneol, and p-cymene-8-ol. The bioconversion products of beta -pinene were the same except for the presence of fenchyl alcohol and the absence of camphor. Most of the accumulated products were p-menthene derivatives such as p-cymene, limonene, and alpha -terpinolene in both substrates. Total bioconversion was 33.5% of alpha -pinene and 58.8% of beta -pinene. (C) 2001 Elsevier Science Ltd. All rights reserved.
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