期刊
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
卷 491, 期 1-2, 页码 1-7出版社
ELSEVIER
DOI: 10.1016/S1383-5718(00)00133-9
关键词
micronuclei; antioxidant vitamins; radioprotection; human lymphocytes
The protective effect of Vitamins C, E and beta -carotene against gamma -ray-induced DNA damage in human lymphocytes in vitro was investigated. Cultured lymphocytes were exposed to increasing concentration of these vitamins either before or after irradiation with 2Gy of gamma -rays and DNA damage was estimated using micronucleus assay. A radioprotective effect was observed when antioxidant vitamins were added to cultured cells before as well after irradiation; the strongest effect was observed when they were added no later than 1 h after irradiation. The radioprotective effect of vitamins also depended on their concentration; Vitamins C added at low concentration (1 mug/ml) before exposure of the cells to radiation prevented induction of micronuclei. Vitamin E at the concentration above 2 mug/ml decreased the level of radiation-induced micronuclei when compared to the cells irradiated without vitamin treatment. beta -Carotene was effective at all tested concentrations from 1 to 5 mug/ml and reduced the number of micronuclei in irradiated cells. The vitamins had no effect on radiation-induced cytotoxicity as measured by nuclear division index. The radioprotective action of antioxidant Vitamins C, E and beta -carotene was dependent upon their concentration as well as time and sequence of application. (C) 2001 Elsevier Science B.V. All rights reserved.
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