期刊
JOURNAL OF BIOMATERIALS APPLICATIONS
卷 29, 期 10, 页码 1428-1438出版社
SAGE PUBLICATIONS LTD
DOI: 10.1177/0885328214568799
关键词
Hydrolyzed gelatin peptide; fibroblast; cell bioactivity; antioxidant; bacterial cellulose
资金
- National Science Council [NSC 101-2120-S-197-001, NSC 101-2313-B-197-002-MY3]
The hydrolyzed gelatin peptides, obtained from the hydrolysis of Tilapia nilotica skin gelatin with alcalase and pronase E, were fractionated using an ultrafiltration system into hydrolyzed gelatin peptides-a (10kDa membrane), hydrolyzed gelatin peptides-b(1), and hydrolyzed gelatin peptides-b(2) (5kDa membrane) fractions. The highest oxygen radical absorbance capacity was observed in hydrolyzed gelatin peptides-b(2), which contained more nonpolar amino acids than the other hydrolyzed gelatin peptides. Hydrolyzed gelatin peptides-b(2) at a concentration of 12.5mg/ml exhibited the highest proliferation ability and increased the expression of Type I procollagen mRNA, which indicated an enhanced collagen synthesis. Hydrolyzed gelatin peptides protected Detroit 551 cells from 2,2-azobis(2-amidinopropane) dihydrochloride-induced oxidative damage and increased cell viability. Hydroxylpropylmethyl cellulose-modified bacterial cellulose and dried fabricated biofilm were less eligible for Detroit 551 cell proliferation than bacterial cellulose. The release of hydrolyzed gelatin peptides in bacterial cellulose film was slower than that in hydroxylpropylmethyl cellulose-modified bacterial cellulose and dried fabricated biofilm; thus, bacterial cellulose film and hydroxylpropylmethyl cellulose-modified bacterial cellulose and dried fabricated biofilm are suitable candidates for applications in delayed release type and rapid release type biofilms, respectively.
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