期刊
FEBS LETTERS
卷 494, 期 1-2, 页码 117-120出版社
WILEY
DOI: 10.1016/S0014-5793(01)02333-X
关键词
phosphatidylinositol-dependent phospholipase C; diacylglycerol; membrane fusion; membrane hemifusion; liposome aggregation
Diacylglycerol increased the hydrolytic activity of phosphatidylinositol-specific phospholipase C on large unilamellar vesicles containing 5-40% phosphatidylinositol. Moreover, diacylglycerol increased the rate and extent of vesicle fusion (contents mixing) induced by the enzyme. Kinetic studies of intervesicular lipid mixing revealed that fusion was limited by the frequency of contacts involving two diacylglycerol-rich domains. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
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