期刊
EUROPEAN JOURNAL OF PHARMACOLOGY
卷 417, 期 1-2, 页码 69-76出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0014-2999(01)00906-2
关键词
5-HT (5-hydroxytryptamine, serotonin) transporter; 5-HT (5-hydroxytryptamine, serotonin) uptake; glia; basic fibroblast growth factor; epidermal growth factor (human)
The aims of this study were to identify monoamine transporters expressed in human glial cells, and to examine the regulation of their expression by stress-related growth factors. The expression of serotonin transporter mRNA was detected by reverse transcriptase-polymerase chain reaction in normal human astrocytes, whereas the dopamine transporter (DAT) and the norepinephrine transporter (NET) were not detected. The cDNA sequence of the glial serotonin transporter in astrocytes was consistent with that reported for the neuronal serotonin transporter (SERT). Moreover, we also demonstrated SEPT expression in glial fibrillary acidic protein-positive cells by immunocytochemical staining in normal human astrocytes. Serotonin transporter gene expression was also detected in glioma-derived cell lines (A172, KG-1-C and KGK). Addition of basic Fibroblast growth factor (bFCF) or epidermal growth factor (EGF) for 2 days increased serotonin transporter gene expression in astrocytes and JAR (human choriocarcinoma cell line). Basic fibroblast growth factor, but not epidermal growth factor, increased specific [H-3]serotonin uptake in astrocytes in a time (1-4 days)- and concentration (20-100 ng/ml)-dependent manner. The expression of genes for basic fibroblast growth factor and epidermal growth factor receptors was detected in astrocytes. These findings suggest that the expression of the serotonin transporter in human glial cells is positively regulated by basic fibroblast growth factor. (C) 2001 Published by Elsevier Science B.V.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据