期刊
BIOCHEMICAL SYSTEMATICS AND ECOLOGY
卷 54, 期 -, 页码 247-256出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bse.2014.02.012
关键词
Genetic variability; Inter-simple sequence repeats (ISSRs); Fagaceae; Quercus
资金
- Portuguese Foundation (FCT) [SFRH/BD/42837/2008]
- IBB-CGB/UTAD
- Fundação para a Ciência e a Tecnologia [SFRH/BD/42837/2008] Funding Source: FCT
The family Fagaceae includes several species and presents huge genetic variability. In the last two decades, several genetic studies about phylogenetics and genetic diversity of Fagaceae have emerged. ISSR markers were used to evaluate the genetic diversity of 26 species of Fagaceae belonging to the genera Castanea, Fagus and Quercus. Among several primers tested, 17 were selected for the evaluation of diversity and estimation of genetic relationships. A total of 371 ISSR markers were produced and each primer revealed high polymorphism. Specific ISSR markers for the Quercus infrageneric groups were amplified. ISSRs proved to be a reliable tool for the discrimination of the analyzed species per genus, infrageneric group and/or ecological origin. (C) 2014 Elsevier Ltd. All rights reserved.
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