Volume-load capacity in fast-gradient liquid chromatography - Effect of sample solvent composition and injection volume on chromatographic performance

We studied the effects of sample solvent composition and injection volumes on the chromatographic performance of ODS-bonded silica columns under fast-gradient running conditions. Chromatographic performance is compromised as a function of both sample injection volume and sample solvent strength: with earlier-eluting analytes being much more affected than later-eluting ems. In general, when injecting samples dissolved in a strong solvent, performance was improved by diluting the strong injection solvent and injecting a proportionally larger volume. Volume loading capacity can be increased by using a longer column, or by using a column of equivalent length, but with a larger inner diameter. Data also suggest that sample solvent strength, not viscosity, is responsible for the noted effects. (C) 2001 Elsevier Science B.V. All rights reserved.