期刊
BIOCHEMICAL SYSTEMATICS AND ECOLOGY
卷 47, 期 -, 页码 126-131出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bse.2012.10.004
关键词
Stipa bungeana; Genetic diversity; Genetic differentiation; SRAP
资金
- Chinese Academy of Sciences [XDA05050202]
- earmarked fund for Modern Agro-industry Technology Research System [CARS-35-40]
In order to investigate the genetic diversity and population structure of Stipa bungeana under the background of grassland utilization and grazing exclusion, ten S. bungeana populations were selected from different steppe type in the Loess Plateau of China. Sequence-related amplified polymorphism (SRAP) marker was used to assess the genetic diversity. Fifteen SRAP primer combinations generated a total of 482 amplification bands, 418 (86.72%) were polymorphic bands. A relatively high level of genetic diversity (PPB = 89.80%, h = 0.1972, H = 0.3154) was detected at the species level, but the genetic diversity was low at the population level (PPB = 17.01-33.33%, h = 0.0438-0.0967, H = 0.0361-0.1447). AMOVA analysis revealed a high level of genetic differentiation among populations (Phi(ST) = 0.6757), and a limited among-population gene flow (N-m = 0.1200). There was no significant correlation between genetic distance and geographic distance by Mantel test (r = 0.1126, P = 0.204). Conservation implications were proposed for S. bungeana. (C) 2012 Elsevier Ltd. All rights reserved.
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