期刊
SENSORS AND ACTUATORS B-CHEMICAL
卷 74, 期 1-3, 页码 100-105出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/S0925-4005(00)00717-6
关键词
foodborne pathogens; surface plasmon resonance; immunosensors
Salmonella enteritidis and Listeria monocytogenes were detected in real time using a surface plasmon resonance (SPR) sensor based on prism excitation of surface plasmons and spectral interrogation. The respective antibodies against the pathogens were immobilized on the gold sensor surface as a covalently crosslinked double-layer or covalently bound on a cross-linked albumin layer. Salmonella and Listeria could be detected by the sensor at concentrations down to 10(6) cell/ml. The sensor sensitivity was comparable with that of standard ELISA in which the same antibodies were used. (C) 2001 Elsevier Science B.V. All rights reserved.
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