期刊
JOURNAL OF CHROMATOGRAPHY B
卷 754, 期 1, 页码 113-119出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0378-4347(00)00588-0
关键词
3 '-C-ethynylcytidine; ion suppression; electrospray ionization
During the development of the liquid chromatography with electrospray ionization-tandem mass spectrometry for the quantitative determination of 3 ' -C-ethynylcytidine (I) in rat plasma, ion suppression caused by the matrix components was observed for I and its structural analogue, 3 ' -C-ethylcytidine (II) as the internal standard. In the method initially designed, I/II peak area ratios varied according to the degree of matrix effect, which led to the poor precision of the assay. From the examination of the ion suppression behavior for I and II, it was assumed that this phenomenon is attributed to the difference in the retention time between I and II. Based on this assumption, therefore, the methanol content in the mobile phase was changed from 5 to 25% so as to make I and II the same retention time. As a result of this modification of the initial method, the precision expressed as relative standard deviation was improved from 5.2-16.2 to 2.7-4.2% in intra-assay and from 6.8-14.9 to 3.5-7.2% in inter-assay validations. (C) 2001 Elsevier Science BN. All rights reserved.
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