期刊
BIOCHEMICAL SYSTEMATICS AND ECOLOGY
卷 38, 期 3, 页码 292-299出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bse.2010.02.003
关键词
Carbon flux; Colony formation; Glyoxylate; Growth; Polysaccharide; Scenedesmus obliquus
资金
- Natural Science Foundation of Jiangsu Province [BK2007743]
- National Natural Science Foundation of China [30970500, 30670404]
To investigate the response of Scenedesmus obliquus to changes in metabolic carbon flux, S. obliquus was cultured in medium with different concentrations of glyoxylate over 9 days. Results showed that growth rates were not affected in the lower concentration glyoxylate (0.25 and 0.5 mM). However, growth rate of S. obliquus was inhibited in the higher concentration glyoxylate (0.85 and 1.25 mM) during the early phase before recovering at higher densities. Changes in growth rates in different glyoxylate concentrations were in line with changes in F-v/F-m and Phi(PSII). Colony formation was observed in S. obliquus in the four glyoxylate treatments. As a consequence, the mean number of cells per particle of S. obliquus in the glyoxylate treatments were significantly higher than those in the control. The total polysaccharide content of S. obliquus cells increased with increased glyoxylate concentrations. The increased glyoxylate-stimulated polysaccharide levels were directly correlated with colony size of S. obliquus. (C) 2010 Elsevier Ltd. All rights reserved.
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