期刊
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
卷 98, 期 9, 页码 5353-5358出版社
NATL ACAD SCIENCES
DOI: 10.1073/pnas.061038298
关键词
Ricinus communis; papain-type KDEL peptidase
The ricinosome (synonym, precursor protease vesicle) is a novel organelle, found so far exclusively in plant cells. Electron microscopic studies suggest that it buds off from the endoplasmic reticulum in senescing tissues. Biochemical support for this unusual origin now comes from the composition of the purified organelle, which contains large amounts of a 45-kDa cysteine endoprotease precursor with a C-terminal KDEL motif and the endoplasmic reticulum lumen residents BiP (binding protein) and protein disulfide isomerase, Western blot analysis, peptide sequencing, and mass spectrometry demonstrate retention of KDEL in the protease preform. Acidification of isolated ricinosomes causes castor bean cysteine endopeptidase activation, with cleavage of the N-terminal propeptide and the C-terminal KDEL motif, We propose that ricinosomes accumulate during senescence by programmed cell death and are activated by release of protons from acidic vacuoles.
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