期刊
JOURNAL OF CHROMATOGRAPHY B
卷 754, 期 2, 页码 477-486出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0378-4347(01)00037-8
关键词
isoniazid; ethionamide; pyrazinamide; morphazinamide; p-aminosalicylic acid
New methods of ion interaction reagent (IIR) RP-HPLC are presented for the determination of anti-tuberculosis drugs and their metabolites, singly or in multi-component mixtures, in biological fluids. The following analytes are considered: isoniazid, ethionamide, pyrazinamide, morphazinamide, p-aminosalicylic acid, nicotinic and isonicotinic acids. Aqueous solutions of three different ion interaction reagents are alternatively or comparatively used as the mobile phases, namely: (A) 5.00 mM octylamine at pH 3.00 for o-phosphoric acid, (B) 5.00 mM octylamine at pH 8.00 for o-phosphoric acid, and (C) 5.00 mM 1,6 diaminohexane at pH 6.00 for o-phosphoric acid. The response linearity between peak area and analyte concentration is verified fur all the analytes in the concentration range within the determination limits and 2.00 mg/l, Detection limits are always lower than 82 mug/l for standard solutions; in the analysis of samples of rat serum, rat plasma and human serum, the matrix effect is negligible, the detection limits are always lower than 94 mug/l and the average recovery yield is always greater than 96%. (C) 2001 Elsevier Science B.V. All rights reserved.
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