期刊
JOURNAL OF MOLECULAR BIOLOGY
卷 308, 期 2, 页码 123-129出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1006/jmbi.2001.4602
关键词
protein folding; folding kinetics; folding nucleus; protein evolution; evolutionary conservation
Here, we present statistical analysis of conservation profiles in families of homologous sequences for nine proteins whose folding nucleus was determined by protein engineering methods. We show that in all but one protein (AcP) folding nucleus residues are significantly more conserved than the rest of the protein. Two aspects of our study are especially important: (i) grouping of amino acid residues into classes according to their physical-chemical properties and (ii) proper normalization of amino acid probabilities that reflects the fact that evolutionary pressure to conserve some amino acid types may itself affect concentration of various amino acid types in protein families. Neglect of an; of those two factors may make physical and biological signals from conservation profiles disappear. (C) 2001 Academic Press.
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