期刊
BIOCHEMICAL SOCIETY TRANSACTIONS
卷 41, 期 -, 页码 1260-1264出版社
PORTLAND PRESS LTD
DOI: 10.1042/BST20130125
关键词
laser; spectroscopy; synchrotron; time-resolved spectroscopy; X-ray crystallography
资金
- Engineering and Physical Sciences Research Council (EPSRC) [EP/I01974X/1]
- BBSRC [BB/H001905/1] Funding Source: UKRI
- EPSRC [EP/I01974X/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/H001905/1] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [EP/I01974X/1] Funding Source: researchfish
To understand the mechanism of biological processes, time-resolved methodologies are required to investigate how functionality is linked to changes in molecular structure. A number of spectroscopic techniques are available that probe local structural rearrangements with high temporal resolution. However, for macromolecules, these techniques do not yield an overall high-resolution description of the structure. Time-resolved X-ray crystallographic methods exist, but, due to both instrument availability and stringent sample requirements, they have not been widely applied to macromolecular systems, especially for time resolutions below 1 s. Recently, there has been a resurgent interest in time-resolved structural science, fuelled by the recognition that both chemical and life scientists face many of the same challenges. In the present article, we review the current state-of-the-art in dynamic structural science, highlighting applications to enzymes. We also look to the future and discuss current method developments with the potential to widen access to time-resolved studies across discipline boundaries.
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