期刊
ANALYTICAL CHEMISTRY
卷 73, 期 9, 页码 2018-2021出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac001227f
关键词
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资金
- NHGRI NIH HHS [HG01389] Funding Source: Medline
A novel thermocycling machine based on a microcapillary equipped with bidirectional pressure-driven flow and in situ optical position sensors is described. A 1-muL droplet of reaction mixture moves between three heat zones in a 1-mm-i.d., oil-filled capillary using a multielement scattered light detector and active feedback. Dwell times and accelerations can be adjusted independently. As a demonstration of the device, 30 cycles of a 500-base pair product were performed in 23 min with 78% amplification efficiency. This result compares well with previous high-speed thermocyclers. Theoretically,, the arrangement can approach a time of 2.5 min for 30 cycle amplifications of a 500-base pair produce.
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