期刊
JOURNAL OF GENERAL PHYSIOLOGY
卷 117, 期 5, 页码 491-503出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1085/jgp.117.5.491
关键词
potassium channel; gating; heteromultimer; I-h; cAMP
类别
资金
- NINDS NIH HHS [R01 NS-36658, R01 NS036658] Funding Source: Medline
Members of the HCN channel family generate hyperpolarization-activated cation currents (I-h) that are directly regulated by cAMP and contribute to pacemaker activity in heart and brain. The four HCN isoforms show distinct but overlapping patterns of expression in different tissues. Here, we report that HCN1 and HCN2, isoforms coexpressed in neocortex and hippocampus that differ markedly in their biophysical properties, coassemble to generate heteromultimeric channels with novel properties. When expressed in Xenopus oocytes, HCN1 channels activate 5-10-fold more rapidly than HCN2 channels. HCN1 channels also activate at voltages that are 10-20 mV more positive than those required to activate HCN2. In cell-free patches, the steady-state activation curve of HCN1 channels show a minimal shift in response to cAMP (+4 mV), whereas that of HCN2 channels shows a pronounced shift (+17 mV). Coexpression of HCN1 and HCN2 yields I-h currents that activate with kinetics and a voltage dependence that tend to be intermediate between those of HCN1 and HCN2 homomers, although the coexpressed channels do show a relatively large shift by cAMP (+14 mV). Neither the kinetics, steady-state voltage dependence, nor cAMP dose-response curve for the coexpressed I-h can be reproduced by the linear sum of independent populations of HCN1 and HCN2 homomers. These results are most simply explained by the formation of heteromeric channels with novel properties. The properties of these heteromeric channels closely resemble the properties of I-h in hippocampal CA1 pyramidal neurons, cells that coexpress HCN1 and HCN2. Finally, differences in I-h channel properties recorded in cell-free patches versus intact oocytes are shown to be due, in part, to modulation of I-h by basal levels of cAMP in intact cells.
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