期刊
BIOCHEMICAL SOCIETY TRANSACTIONS
卷 40, 期 -, 页码 698-703出版社
PORTLAND PRESS LTD
DOI: 10.1042/BST20120050
关键词
Alzheimer's disease; nuclear magnetic resonance (NMR); phosphorylation; protein kinase A; tau protein
资金
- Agence Nationale de la Recherche [ANR-05 blanc-6320-01]
- Region Nord
- CNRS (Centre National de la Recherche Scientifique) Pasteur Institute of Lille
- French Research Ministry
- University of Sciences and Technologies of Lille I
- CNRS/Region Nord-Pas de Calais (France)
- Tres Grands Equipements Resonance Magnetique Nucleaire Tres Hauts Champs (TGE RMN THC) [FR-3050]
We describe our efforts to combine in vitro enzymatic reactions with recombinant kinases to phosphorylate the neuronal tau protein, and NMR spectroscopy to unravel the resulting phosphorylation pattern in both qualitative and quantitative manners. This approach, followed by functional assays with the same samples, gives access to the complex phosphorylation code of tau. As a result, we propose a novel hypothesis for the link between tau (hyper)phosphorylation and aggregation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据