期刊
BIOCHEMICAL SOCIETY TRANSACTIONS
卷 39, 期 -, 页码 77-81出版社
PORTLAND PRESS LTD
DOI: 10.1042/BST0390077
关键词
central carbohydrate metabolism; crenarchaeon; Entner-Doudoroff pathway; glucose dehydrogenase; glycolysis; Sulfolobus solfataricus; Sulfobbus Systems Biology (SulfoSYS)
资金
- Federal Ministry of Education and Research (BMBF) Germany [SysMo P-N-01-09-23]
Within the SulfoSYS (Sulfolobus Systems Biology) project, the effect of temperature on a metabolic network is investigated at the systems level. Sulfolobus solfataricus utilizes an unusual branched ED (Entner-Doudoroff) pathway for sugar degradation that is promiscuous for glucose and galactose. In the course of metabolic pathway reconstruction, a glucose dehydrogenase isoenzyme (GDH-2, 5503204) was identified. GDH-2 exhibits high similarity to the previously characterized GDH-1 (S503003, 61% amino acid identity), but possesses different enzymatic properties, particularly regarding substrate specificity and catalytic efficiency. In contrast with GDH-1, which exhibits broad substrate specificity for C-5 and C-6 sugars, GDH-2 is absolutely specific for glucose. The comparison of kinetic parameters suggests that GDH-2 might represent the major player in glucose catabolism via the branched ED pathway, whereas GDH-1 might have a dominant role in galactose degradation via the same pathway as well as in different sugar-degradation pathways.
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