4.4 Article

Structure and function of the primase domain of the replication protein from the archaeal plasmid pRN1

期刊

BIOCHEMICAL SOCIETY TRANSACTIONS
卷 39, 期 -, 页码 104-106

出版社

PORTLAND PRESS LTD
DOI: 10.1042/BST0390104

关键词

archaeon; plasmid; polymerase; primase; pRN1

资金

  1. Deutsche Forschungsgemeinschaft [Li913/4, Li913/6]

向作者/读者索取更多资源

The replication protein of the archaeal plasmid pRN1 is a multifunctional enzyme which appears to carry out several steps at the plasmid replication initiation. We recently determined the structure of the minimal primase domain of the replication protein and found out that the primase domain consists of a catalytic primase/polymerase domain and an accessory helix-bundle domain. Structure-guided mutagenesis allowed us to identify amino acids which are important for template binding, dinucleotide formation and a step before primer extension. On the basis of functional and structural data, we propose a model of the catalytic cycle of primer synthesis by the pRN1 replication protein.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.4
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据