4.4 Article

CRISPR-based immune systems of the Sulfolobales: complexity and diversity

期刊

BIOCHEMICAL SOCIETY TRANSACTIONS
卷 39, 期 -, 页码 51-57

出版社

PORTLAND PRESS LTD
DOI: 10.1042/BST0390051

关键词

archaeal virus; cluster of regularly interspaced palindromic repeats/Cas module; (CRISPR/Cas module); Cmr module; CRISPR RNA (crRNA); protospacer-associated motif (PAM)

资金

  1. Danish Natural Science Research Council [272-08-0391]
  2. Danish Research Council for Technology and Production [274-07-0116]
  3. Danish National Research Foundation

向作者/读者索取更多资源

CRISPR (cluster of regularly interspaced palindronnic repeats)/Cas and CRISPR/Cmr systems of Sulfolobus, targeting DNA and RNA respectively of invading viruses or plasmids are complex and diverse. We address their classification and functional diversity, and the wide sequence diversity of RAMP (repeat-associated mysterious protein)-motif containing proteins encoded in Cmr modules. Factors influencing maintenance of partially impaired CRISPR-based systems are discussed. The capacity for whole CRISPR transcripts to be generated despite the uptake of transcription signals within spacer sequences is considered. Targeting of protospacer regions of invading elements by (as protein-crRNA (CRISPR RNA) complexes exhibit relatively low sequence stringency, but the integrity of protospacer-associated motifs appears to be important. Different mechanisms for circumventing or inactivating the immune systems are presented.

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