期刊
BIOCHEMICAL SOCIETY TRANSACTIONS
卷 39, 期 -, 页码 629-634出版社
PORTLAND PRESS LTD
DOI: 10.1042/BST0390629
关键词
bis-amino-U (BAU); DNA recognition; nucleotide analogue; oligonucleotide; triple-helix formation
资金
- Biotechnology and Biological Sciences Research Council [BB/D005981/1] Funding Source: researchfish
- BBSRC [BB/D005981/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/D005981/1] Funding Source: Medline
Triple-helical nucleic acids are formed by binding an oligonucleotide within the major groove of duplex DNA. These complexes offer the possibility of designing oligonucleotides which bind to duplex DNA with considerable sequence specificity. However, triple-helix formation with natural nucleotides is limited by (i) the requirement for low pH, (ii) the requirement for homopurine target sequences, and (iii) their relatively low affinity. We have prepared modified oligonucleotides to overcome these limitations, including the addition of positive charges to the sugar and/or base, the inclusion of cytosine analogues, the development of nucleosides for recognition of pyrimidine interruptions and the attachment of one or more cross-linking groups. By these means we are able to generate triplexes which have high affinities at physiological pH at sequences that contain pyrimidine interruptions.
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