期刊
JOURNAL OF EXPERIMENTAL BOTANY
卷 52, 期 358, 页码 1071-1081出版社
OXFORD UNIV PRESS
DOI: 10.1093/jexbot/52.358.1071
关键词
GS1; tobacco; Nicotiana tabacum; transgenic; ammonia assimilation
Nitrogen, which is a major limiting nutrient for plant growth, is assimilated as ammonium by the concerted action of glutamine synthetase (GS) and glutamate synthase (GOGAT), GS catalyses the critical incorporation of inorganic ammonium into the amino acid glutamine. Two types of GS isozymes, located in the cytosol (GS(1)) and in the chloroplast (GS(2)) have been identified in plants. Tobacco (Nicotiana tabacum) transformants, over-expressing GS(1) driven by the constitutive CaMV 35S promoter were analysed. GS in leaves of GS-5 and GS-8 plants was up-regulated, at the level of RNA and proteins. These transgenic plants had six times higher leaf GS activity than controls. Under optimum nitrogen fertilization conditions there was no effect of GS over-expression on photosynthesis or growth. However, under nitrogen starvation the GS transgenics had c, 70% higher shoot and c, 100% greater root dry weight as well as 50% more leaf area than low nitrogen controls. This was achieved by the maintenance of photosynthesis at rates indistinguishable from plants under high nitrogen, while photosynthesis in control plants was inhibited by 40-50% by nitrogen deprivation. It was demonstrated that manipulation of GS activity has the potential to maintain crop photosynthetic productivity while reducing nitrogen fertilization and the concomitant pollution.
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