Translation termination in human mitochondrial ribosomes

Mitochondria are ubiquitous and essential organelles for all nucleated cells of higher eukaryotes They contain their own gi nome [mtDNA (mitochondrial DNA)] and this autosomally replicating extranuclear DNA encodes a complement of genes whose products are required to couple oxidative phosphorylation Sequencing of this human mtDNA more than 20 years ago revealed unusual features that included a modified codon usage Specific deviations from the standard genetic code include recoding of the conventional UGA stop to tryptophan and strikingly the apparent recoding of two arginine triplets (AGA and AGG) to termination signals This latter reassignment was made because of the absence of cognate mtDNA encoded tRNAs and a lack of tRNAs imported from the cytosol Each of these codons only occurs once and in both cases at the very end of an open reading frame The presence of both AGA and AGG is rarely found in other mammals and the molecular mechanism that has driven the change from encoding arginine to dictating a translational stop has posed a challenging conundrum Mitochondria from the majority of other organisms studied use only UAA and UAG leaving the intriguing question of why human organelles appear to have added the complication of a further two stop codons AGA and AGG or have they? In the present review we report recent data to show that mammalian mitochondria can utilize a 1 frameshift such that only the standard UAA and UAG stop codons are required to terminate the synthesis of all 13 polypeptides