期刊
PLANT AND CELL PHYSIOLOGY
卷 42, 期 5, 页码 475-481出版社
OXFORD UNIV PRESS
DOI: 10.1093/pcp/pce060
关键词
Capsicum annuum; cell wall; hydroxycinnamoyl-CoA : tyramine; N-(hydroxycinnamoyl)transferase; UV-C treatment; N-(hydroxycinnamoyl)-amines
Hydroxycinnamoyl-CoA : tyramine N-(hydroxycinnamoyl) transferase (THT) is a pivotal enzyme in the synthesis of N-(hydroxycinnamoyl)-amines, which are associated with cell wall fortification in plants. The cDNA encoding THT was cloned from the leaves of UV-C treated Capsicum annuum (hot pepper) using a differential screening strategy. The predicted protein encoded by the THT cDNA is 250 amino acids in length and has a relative molecular mass of 28,221, The protein sequence derived from the cDNA shares 76% and 67% identity with the potato and tobacco THT protein sequences, respectively, The recombinant pepper THT enzyme was purified using a bacterial overexpression system, The purified enzyme has a broad substrate specificity including acyl donors such as cinnamoyl-, sinapoyl-, feruloyl-, caffeoyl-, and 4-coumaroyl-CoA and accepters such as tyramine and octopamine. In UV-C treated plants, the THT mRNA was strongly induced in leaves, and the elevated level of expression was stable for up to 36 h, THT mRNA also Increased in leaves that were detached from the plant but not treated with UV-C, THT expression was measured in different plant tissues, and was constitutive at a similar level in leaf, root, stem, flower and fruit. Induction of TWT mRNA was correlated with an increase in THT protein.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据