Production, purification, and characterization of an intracellular aflatoxin-detoxifizyme from Armillariella tabescens (E-20)

Some Armillariella tabescens (E-20) multienzymes have previously been reported to present detoxifying activities against aflatoxins. In this paper, we describe the isolation purification of an intracellular enzyme, named aflatoxin-detoxifizyme, which exhibited detoxification activity on aflatoxin B-1 (AFB(1)). This aflatoxin-detoxifizyme exhibited a specific activity of 7.09 nmol min/mg at pH 6.0 and 28 degreesC. The apparent molecular mass was 51.8 kDa as determined by SDS-PAGE. The isoelectric point was estimated to be 5.4 and optimum activity for the enzyme was found at pH 6.8 and 35 degreesC. The activity of the purified enzyme was confirmed by Ames test. (C) 2001 Elsevier Science Ltd. All rights reserved.