期刊
MOLECULAR MICROBIOLOGY
卷 40, 期 4, 页码 869-878出版社
WILEY
DOI: 10.1046/j.1365-2958.2001.02426.x
关键词
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Procyclins are the major surface glycoproteins of insect-form Trypanosoma brucei. The procyclin expression sites are polycistronic and are transcribed by an alpha -amanitin-resistant polymerase, probably RNA polymerase I (Pol I). The expression sites are flanked by transcription units that are sensitive to alpha -amanitin, which is a hallmark of pol II-driven transcription. We have analysed a region of 9.5 kb connecting the EP/PAG2 expression site with the downstream transcription unit. The procyclin expression site is longer than was previously realized and contains an additional gene, procyclin-associated gene 4 (PAG4), and a region of unknown function, the T region, that gives rise to trans-spliced, polyadenylated RNAs containing small open reading frames (ORFs), Two new genes, GU1 and GU2, were identified in the downstream transcription unit on the opposite strand. Unexpectedly, the 3' untranslated region of GU2 and the complementary T transcripts overlap by several hundred base pairs. Replacement of GU2 by a unique tag confirmed that sense and antisense transcription occurred from a single chromosomal locus. Overlapping transcription is stage specific and may extend greater than or equal to 10 kb in insect-form trypanosomes, The nucleotide composition of the T brucei genome Is such that antisense ORFs occur frequently, If stable mRNAs can be derived from both strands, the coding potential of the genome may be substantially larger than has previously been suspected.
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