期刊
INTERNATIONAL JOURNAL OF CANCER
卷 92, 期 3, 页码 361-369出版社
WILEY-LISS
DOI: 10.1002/ijc.1202
关键词
VEGF; anti-sense RNA; pancreatic cancer; angiogenesis; tumorigenicity
类别
资金
- NCI NIH HHS [CA-40162] Funding Source: Medline
Vascular endothelial growth factor (VEGF) is a potent angiogenic stimulator that acts by binding to high-affinity transmembrane receptors, Although both VEGF and its receptors are overexpressed in human pancreatic ductal adenocarcinoma (PDAC), this malignancy is not generally considered to be highly vascular. It is not known, therefore, whether the abundance of VEGF in PDAC is biologically relevant, To address this issue, we measured the angiogenic effects of pancreatic cancer cell-derived VEGF in an in vitro endothelial cell proliferation assay and characterized the consequences of suppressing VEGF expression on pancreatic tumor growth in an athymic nude mouse model. We found that human pancreatic cancer cell lines secrete large quantities of biologically active VEGF into conditioned medium (GM), Stable transfection of an anti-sense VEGF(189) (AS-VEGF(189)) expression construct: into PANG-I pancreatic cancer cells resulted in decreased VEGF expression and secretion, a decreased capacity of the resultant GM to enhance endothelial cell proliferation and a significant attenuation of tumor cell proliferation in vitro. Furthermore. when injected into athymic nude mice, AS-VEGF(189)-expressing cells exhibited an 80% decrease in tumor growth compared with control cells, These results support the hypothesis that VEGF promotes pancreatic cancer growth in vivo and suggest that anti-VEGF therapy may be useful in the treatment of this disease. (C) 2001 Wiley-Liss. Inc.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据