Preparation of chlorin e6-monoclonal antibody conjugate and its effect for photodynamic therapy

Chlorin e6 (Ce6) was conjugated with anti-tumor monoclonal antibody (IgG2b) to increase its binding affinity for tumors. The Ce6 was activated by N-hydroxysuccinimide and directly conjugated with IgG2b (IgG2b-Ce6) via peptide bonds. An average of 11 Ce6 molecules were coupled to each IgG2b molecule. IgG2b-Ce6 showed high cellular uptake in tumor cells and high photocytotoxicity. These results indicate IgG2b-Ce6 is a good photosensitizer with high binding affinity to tumor cells. Copyright (C) 2001 John Wiley & Sons, Ltd.