期刊
JOURNAL OF BACTERIOLOGY
卷 183, 期 10, 页码 3256-3260出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JB.183.10.3256-3260.2001
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- NIGMS NIH HHS [GM50577] Funding Source: Medline
Tn5 insertion mutants defective in regulation of the Pseudomonas putida trpE and trpGDC operons by tryptophan were found to contain insertions in the P. putida mia 4 gene, whose product (in Escherichia coli) modifies tRNA(Trp) and is required for attenuation. Nucleotide sequences upstream of trpE and trpG encode putative leader peptides similar in sequence to leader peptides found in other bacterial species, and the phenotypes of the mutants strongly suggest Chat transcription of these operons is regulated solely by attenuation.
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