期刊
ANALYTICAL CHEMISTRY
卷 73, 期 9, 页码 1917-1926出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac0013709
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资金
- NIGMS NIH HHS [GM59240] Funding Source: Medline
MALDI-quadrupole time-of-flight mass spectrometry was applied to identify proteins from organisms whose genomes are still unknown. The identification was carried out by successively searching a sequence database-first with a peptide mass fingerprint, then with a packet of noninterpreted MS/MS spectra, and finally with peptide sequences obtained by automated interpretation of the MS/MS spectra. A MS BLAST homology searching protocol Nas developed to overcome specific limitations imposed by mass spectrometric data, such as the limited accuracy of de novo sequence predictions. This approach was tested in a small-scale proteomic project involving the identification of 15 bands of gel-separated proteins from the methylotrophic yeast Pichia pastoris, whose genome has not yet been sequenced and which is only distantly related to other fungi.
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