4.5 Article

Occurrence and subcellular distribution of the NAD(P)HX repair system in mammals

期刊

BIOCHEMICAL JOURNAL
卷 460, 期 -, 页码 49-58

出版社

PORTLAND PRESS LTD
DOI: 10.1042/BJ20131482

关键词

dehydration; epimerization; glyceraldehyde-3-phosphate dehydrogenase (GAPDH); metabolite repair; NADHX; NADPHX

资金

  1. FNRS (Fonds de la Recherche Scientifique)
  2. Welbio (Walloon Region)
  3. Interuniversity Attraction Poles Program [P7/43]
  4. European Union [2768141]
  5. U.S. National Science Foundation [MCB-1153413]
  6. Direct For Biological Sciences
  7. Div Of Molecular and Cellular Bioscience [1153413] Funding Source: National Science Foundation

向作者/读者索取更多资源

Hydration of NAD(P)H to NAD(P)HX, which inhibits several dehydrogenases, is corrected by an ATP-dependent dehydratase and an epimerase recently identified as the products of the vertebrate Carkd (carbohydrate kinase domain) and Aibp (apolipoprotein AI-binding protein) genes respectively. The purpose of the present study was to assess the presence of these enzymes in mammalian tissues and determine their subcellular localization. The Carkd gene encodes proteins with a predicted mitochondrial propeptide (mCARKD), a signal peptide (spCARKD) or neither of them (cCARKD). Confocal microscopy analysis of transfected CHO (Chinese-hamster ovary) cells indicated that cCARKD remains in the cytosol, whereas mCARKD and spCARKD are targeted to the mitochondria and the endoplasmic reticulum respectively. Unlike the other two forms, spCARKD is N-glycosylated, supporting its targeting to the endoplasmic reticulum. The Aibp gene encodes two different proteins, which we show to be targeted to the mitochondria (mAIBP) and the cytosol (cAIBP). Quantification of the NAD(P)HX dehydratase and epimerase activities in rat tissues, performed after partial purification, indicated that both enzymes are widely distributed, with total activities of approximate to 3-10 nmol/min per g of tissue. Liver fractionation by differential centrifugation confirmed the presence of the dehydratase and the epimerase in the cytosol and in mitochondria. These data support the notion that NAD(P)HX repair is extremely widespread.

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