期刊
BIOCHEMICAL JOURNAL
卷 460, 期 -, 页码 353-361出版社
PORTLAND PRESS LTD
DOI: 10.1042/BJ20140003
关键词
astrocyte; fluorescence resonance energy transfer (FRET); glucose; glycolytic ATP; mitochondrial ATP; Na+/K+-ATPase
资金
- Fondo Nacional de Desarrollo Cientifico y Tecnologico (FONDECYT) [1010936, 1130095]
- Chilean Government through the Centers of Excellence Base Financing Program of Comision Nacional de Investigacion Cientifica y Tecnologica (CONICYT)
There is abundant evidence that glycolysis and theNa(+)/K+-ATPase pump are functionally coupled, and it is thought that the nature of the coupling is energetic, with glycolysis providing the ATP that fuels the pump. This notion has been instrumental to current models of brain energy metabolism. However, structural and biophysical considerations suggest that the pump should also have access to mitochondrial ATP, which is much more abundant. In the present study, we have investigated the source of ATP that fuels the Na+ pump in astrocytes, taking advantage of the high temporal resolution of recently available FRET nanosensors for glucose, lactate and ATP. The activity of the Na+ pump was assessed in parallel with the Na+-sensitive dye SBFI AM (Na+-binding benzofuran isophthalate acetoxymethyl ester). OXPHOS (oxidative phosphorylation) inhibition resulted in bulk ATP depletion and a 5-fold stimulation of glycolytic flux, in spite of which Na+ pumping was inhibited by 90%. Mathematical modelling of ATP dynamics showed that the observed pump failure is inconsistent with preferential fuelling of the Na+ pump by glycolytic ATP. We conclude that the nature of the functional coupling between the Na+ pump and the glycolytic machinery is not energetic and that the pump is mainly fuelled by mitochondrial ATP.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据