期刊
BIOCHEMICAL JOURNAL
卷 449, 期 -, 页码 555-566出版社
PORTLAND PRESS LTD
DOI: 10.1042/BJ20121262
关键词
amino acid transport; Leishmania; osmoregulation; parasitic protozoa
资金
- Israel Science Foundation
- Academy of Sciences and Humanities [402/08]
- Swiss National Science Foundation [CRSII3 127300]
- Swiss National Science Foundation (SNF) [CRSII3_127300] Funding Source: Swiss National Science Foundation (SNF)
Unlike all other organisms, parasitic protozoa of the family Trypanosomatidae maintain a large cellular pool of proline that, together with the alanine pool, serve as alternative carbon sources as well as reservoirs of organic osmolytes. These reflect adaptation to their insect vectors whose haemolymphs are exceptionally rich in the two amino acids. In the present study we identify and characterize a new neutral amino acid transporter, LdAAP24, that translocates proline and alanine across the Leishmania donovani plasma membrane. This transporter fulfils multiple functions: it is the sole supplier for the intracellular pool of proline and contributes to the alanine pool; it is essential for cell volume regulation after osmotic stress; and it regulates the transport and homoeostasis of glutamate and arginine, none of which are its substrates. Notably, we provide evidence that proline and alanine exhibit different roles in the parasitic response to hypotonic shock; alanine affects swelling, whereas proline influences the rate of volume recovery. On the basis of our data we suggest that LdAAP24 plays a key role in parasite adaptation to its varying environments in host and vector, a phenomenon essential for successful parasitism.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据