期刊
MICROBIOLOGY-SGM
卷 147, 期 -, 页码 1189-1195出版社
MICROBIOLOGY SOC
DOI: 10.1099/00221287-147-5-1189
关键词
beta-galactosidase; yeast; fungal pathogen; gene regulation
类别
资金
- NIGMS NIH HHS [GM37049] Funding Source: Medline
The study of gene regulation in many organisms has been facilitated by the development of reporter genes. The authors report the use of lacZ from Streptococcus thermophilus, a gene encoding a beta -galactosidase, as a reporter for the fungal pathogen Candida albicans. As test cases, Strep. thermophilus lacZ was placed under control of three different C. albicans promoters: MAL2 (maltase), inducible by maltose; HWP1 (hyphal cell wall protein), induced by conditions that promote filamentous growth; and ACT1 (actin). These constructs were each integrated into the C. albicans genome and beta -galactosidase activity was readily detected from these strains, but only under the appropriate growth conditions. beta -Galactosidase activity could be detected by several methods: quantitative liquid assays using permeabilized cells. colorimetric assays of colonies replicated to paper filters, and in situ coloration of colonies growing on medium containing the indicator X-Gal. These results show the usefulness of Strep. thermophilus lacZ as a monitor of gene regulation in this medically important yeast.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据