期刊
BIOCHEMICAL JOURNAL
卷 449, 期 -, 页码 295-305出版社
PORTLAND PRESS LTD
DOI: 10.1042/BJ20121240
关键词
casein kinase 2 (CK2); cystic fibrosis; cystic fibrosis transmembrane-conductance regulator (CFTR); fragmentation; Delta F508-CFTR mutation; F508delCFTR
资金
- Wellcome Trust [088929, 069150]
- FCT (Fundacao para a Ciencia e a Tecnologia) [PTDC/BIA-BCM/112635/2009, PEst-OE/BIA/U14046/2011, SFRH/BD/47445/2008]
- Fondazione per la Ricerca sulla Fibrosi Cistica [3/2011]
- Fundação para a Ciência e a Tecnologia [PTDC/BIA-BCM/112635/2009, SFRH/BD/47445/2008] Funding Source: FCT
Most CF (cystic fibrosis) results from deletion of a phenylalanine (F-508) in the CFTR {CF transmembrane-conductance regulator; ABCC7 [ABC (ATP-binding cassette) sub-family C member 7]} which causes ER (endoplasmic reticulum) degradation of the mutant. Using stably CFTR-expressing BHK (baby-hamster kidney) cell lines we demonstrated that wild-type CTIR and the F508delCFTR mutant are cleaved into differently sized N- and C-terminal-bearing fragments, with each hemi-CFTR carrying its nearest NBD (nucleotide-binding domain), reflecting differential cleavage through the central CFTR R-domain. Similar NBD1-bearing fragments are present in the natively expressing HBE (human bronchial epithelial) cell line. We also observe multiple smaller fragments of different sizes in BHK cells, particularly after F508del mutation (ladder pattern). Trapping wild-type CFT'R in the ER did not generate a F508del fragmentation fingerprint. Fragments change their size/pattern again post-mutation at sites involved in CFTR's in vitro interaction with the pleiotropic protein kinase CK2 (S511A in NBD1). The F508del and S511A mutations generate different fragmentation fingerprints that are each unlike the wild-type; yet, both mutants generate new N-terminal-bearing CFTR fragments that are not observed with other CK2-related mutations (S511D, S422A/D and T1471A/D). We conclude that the F508delCFTR mutant is not degraded completely and there exists a relationship between CFTR's fragmentation fingerprint and the CFTR sequence through putative CK2-interactive sites that lie near F508.
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