期刊
BIOCHEMICAL JOURNAL
卷 438, 期 -, 页码 303-313出版社
PORTLAND PRESS LTD
DOI: 10.1042/BJ20110683
关键词
mucin; multigene family; quantitative PCR; TcSMUG; Trypanosoma cruzi
资金
- UNICEF/World Bank/WHO (World Health Organization)
- ANPCyT (Agencia Nacional de Promocion Cientifica y Tecnologica)
- Fundacion Florencio Fiorini and UNSAM (Universidad National De San Martin)
The surface of the protozoan Trypanosoma cruzi is covered by a dense coat of mucin-type glycoconjugates, which make a pivotal contribution to parasite protection and host immune evasion. Their importance is further underscored by the presence of >1000 mucin-like genes in the parasite genome. In the present study we demonstrate that one such group of genes, termed TcSMUG L, codes for previously unrecognized mucin-type glycoconjugates anchored to and secreted from the surface of insect-dwelling epimastigotes. These features are supported by the in vivo tracing and characterization of endogenous TcSMUG L products and recombinant tagged molecules expressed by transfected parasites. Besides displaying substantial homology to TcSMUG S products, which provide the scaffold for the major Gp35/50 mucins also present in insect-dwelling stages of the T cruzi lifecycle, TcSMUG L products display unique structural and functional features, including being completely refractory to sialylation by parasite trans-sialidases. Although quantitative real time-PCR and gene sequencing analyses indicate a high degree of genomic conservation across the T cruzi species, TcSMUG L product expression and processing is quite variable among different parasite isolates.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据