A thermostable K+-stimulated vacuolar-type pyrophosphatase from the hyperthermophilic bacterium Thermotoga maritima

Current evidence suggests the occurrence of two classes of vacuolar-type H+-translocating inorganic pyrophosphatases (V-PPases): K+-insensitive proteins, identified in eukaryotes, bacteria and archaea, and K+-stimulated V-PPases, identified to date only in eukaryotes, Here, we describe the functional characterization of a thermostable V-PPase from the anaerobic hyperthermophilic bacterium Thermotoga maritima by heterologous expression in Saccharomyces cerevisiae. The activity of this 71-kDa membrane-embedded polypeptide has a near obligate requirement for K+, like the plant V-PPase, and its thermostability depends on the binding of Mg2+, Phylogenetic analysis of protein sequences consistently assigned the T, maritima V-PPase to the K+-sensitive class of V-PPases so far only known for eukaryotes. The finding of a K+-stimulated V-PPase also in a member of a primitive eubacterial lineage strongly supports an ancient evolutionary origin of this group of pyrophosphate-energized proton pumps, (C) 2001 Federation of European Biochemical Societies, Published by Elsevier Science B.V. All rights reserved.